Light and fluorescence microscopy for Life Sciences

  • Teaching

    Details

    Faculty Faculty of Science and Medicine
    Domain Biology
    Code UE-SBL.00125
    Languages English
    Type of lesson Lecture
    Level Master
    Semester SA-2022

    Schedules and rooms

    Summary schedule Monday , Cours bloc (Autumn semester)
    Contact's hours 28

    Teaching

    Responsibles
    Teachers
    Description Fluorescence microscopy has become one of the core techniques in biological research. Its applications range from the study of the expression of specific molecular markers with high spatial resolution in single cells to the probing of cell functions in living organisms. Constant progress in microscope design and in fluorescent probe development has led to a large choice of applications based on the principles of fluorescence microscopy. This course will aim at giving an understanding of key concepts of the main techniques used in life sciences. It will also insist on practical issues essential for a productive use of these techniques in biological and biomedical research.
    Training objectives

    - The fundamental principles of light and fluorescence microscopy
    - The differences between wide-field, confocal and 2-photon microscopy
    - The basics of TIRF, FRET and FRAP 
    - The essential concepts in image acquisition and processing
    - The basics of fluorescence imaging from live samples
    - What types of fluorescent probes are available
    - What super resolution and single molecule imaging can achieve
    - How to image a whole organ at cellular resolution

    Condition of access

    Seating will be assigned in order of registration, until the maximal capacity is reached.

     

    Comments

     

    Moodle page:https://moodle.unifr.ch/course/view.php?id=267639

    Softskills
    No
    Off field
    No
    BeNeFri
    Yes
    Mobility
    Yes
    UniPop
    No
  • Dates and rooms
    Date Hour Type of lesson Place
    28.11.2022 08:15 - 12:00 Cours PER 21, Room E040
    28.11.2022 13:15 - 17:00 Cours PER 17, Room 010
    30.11.2022 08:15 - 12:00 Cours PER 21, Room E040
    30.11.2022 13:15 - 17:00 Cours PER 17, Room 010
    02.12.2022 08:15 - 12:00 Cours PER 17, Room 010
    02.12.2022 13:15 - 17:00 Cours PER 17, Room 010
    05.12.2022 08:15 - 12:00 Cours PER 21, Room E040
    05.12.2022 13:15 - 17:00 Cours PER 21, Room E040
    07.12.2022 08:15 - 12:00 Cours PER 21, Room E040
    07.12.2022 13:15 - 17:00 Cours PER 21, Room B230
  • Assessments methods

    Written exam - SA-2022, Session d'hiver 2023

    Date 10.02.2023 13:15 - 14:00
    Assessments methods By rating
    Descriptions of Exams

    Written exam (45 min.). One mark. 

  • Assignment
    Valid for the following curricula:
    Additional Courses in Sciences
    Version: ens_compl_sciences
    Paquet indépendant des branches > Specialized courses in Biology (Master level)

    Additional programme requirements for PhD studies [PRE-DOC]
    Version: 2020_1/v_01
    Additional programme requirements for PhD studies (Faculty of Science and Medicine) > Specialized courses in Biology (Master level)

    Biology [3e cycle]
    Version: 2015_1/V_01
    Continuing education > Specialized courses in Biology (Master level)

    Biology [POST-DOC]
    Version: 2015_1/V_01
    Continuing education > Specialized courses in Biology (Master level)

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