New users

To start the registration process for new users of flow cytometer (analysers), please download the registration form and the policies, fill it out and send it to us, signed, by email. If you don't know the instrument to use, we will redirect you after discussion on the best instrument for your project needs.

We will contact you to fix a date for the theoretical (1h15) and practical training (1h) on the instrument of interest. 

Once the training is completed and the facility policy is read and signed, the following user rights willl be granted:

• Access to the booking system for a given instrument
• Access to the premises of the instrument (campus card update)
• The right to autonomously use the instruments

Facility users

If you need access to another instrument (flow cytometer analyser), please email your request to us. We will contact you to fix a date for practical training (1h) on the instrument of interest. After completion of the training, you will be granted access to the booking system of the requested instrument.

Booking

To use our cell sorting service for the first time, please download the request form and email the filled out form to us. We will contact you to fix a meeting to discuss your project and your need for the instrument. 

To book the cell sorter for on going project, please check the instrument availability on the booking system before sending us an appointment request accordingly. Please book the instrument 1 week in advance!

If you are not a member of the University of Fribourg, please contact our staff first.

Experiment

The operator will process your samples on the instrument. You are required to stay until the right parameters are set for the sorting of your cells, and to come to pick your cells after sorting for further processing.

Please read carefully the notes for cell sorting to avoid common mistakes!

10x Genomics Chromium Controller

This instrument allow the preparation of single-cell mRNA libraries for NGS. If you plan to use our chromium controller, please contacte us via email. We can discuss your need and how to process. 

We have several level of usage: done for you by our operators, done on our instrument by technician from other NGS facilities, or you can be trained and use it in a semi-autonomeous way. 

QSep100

To access our QSep100 Capillary Electrophoresis system, please contact us via mail. 

We will discuss your project details to determine if you need to be trained and access the instrument in a autonomous way, or if we process your samples for you. 

At UNIFR, the software used for flow cytometry manual analysis is FlowJo. 

If you are a member of the Faculty of Science and Medicine, you can apply for a FlowJO license (limited number, shared licenses). If you are interested, please send an email. We will come back to you with information on how to get and use the license. 

Upon request the facility provide also a beginner (1h) or advanced training (1h30) on FlowJo software. 

Regarding unsupervised analysis of your data, some solution are on trials by our user group. Please contact directly Sarah Cattin for more information.