Instruments

The Cell Analytics Facility provide full access to cutting-edge instruments of flow cytometry. It also contain some instrument to prepare library for NSG. 

Here is the list of the different instruments available in the Cell Analytics Facility at UNIFR. To access these instruments, please follow the process on the Cell Analytics Services page.

How to access instruments

  • Flow Cytometer Analysers

      Details/docs/specs

    • 4 lasers : 405nm (violet 80mW) / 488nm (blue 50mW) / 561nm (yellow-green, 30mW) / 638nm (red 50mW)
    • Simultaneous detection of up to 13 fluorochromes +FSC/SSC
    • Single tube or 96well plate loader options.

     Details/docs/specs

    • 3 lasers : 405nm (violet 50mW) / 488nm (blue 50mW) / 640nm (red 40mW)
    • Simultaneous detection of up to 11 fluorochromes +FSC/SSC
    • Single tube loader

     Details/docs/specs

    • 3 lasers : 405nm (violet 30mW) / 488nm (blue 20mW) / 633nm (red 17mW)
    • Simultaneous detection of up to 8 fluorochromes +FSC/SSC
    • Single tube loader

     Details/docs/specs

    • 5 lasers : 355nm (UV 20mW) / 405nm (Violet 100mW) / 488nm (blue 50mW) / 561nm (Yellow-Green 50mW) / 640nm (red 80mW)
    • Simultaneous detection of many (theoretical maximum of 60) +FSC/2x SSC
    • Single tube loader
    • Soon on site: HTS for multi-tubes or 96wells plates acquisition
  • Flow Cytometer Cell Sorter
    • Our FACSAria Fusion is mainly dedicated to the isolation of eukaryotic nucleated cells. Yeast, bacteria, nanomaterial or any other special applications must be discussed with the facility staff first.
    • Any population of cells inside a more complex heterogeneous-population can be isolated, as long as the cells of interest can be identified in the parental population by light scattering and/or fluorescence (for example: GFP-infected cells or antigen-marker populations using fluoro-labeled antibodies).

       Details/docs/specs

    • 3 lasers : 405nm (violet 85mW) / 488nm (blue 50mW) / 640nm (red 100mW)
    • Simultaneous detection of up to 14 fluorochromes +FSC/SSC
    • Single tube loader
    • Sort of up to 4 independent cell populations in parallel.
    • Sort in 15ml or 5ml tubes, eppendorfs, slides or 384 to 6 wells plates. 
    • Operator used only!
    • Sterile (biosafety cabinet) and BSL-2 samples sort possible.
  • Sample preparation for NGS

       Details/docs/specs

    • For the preparation of single-cell mRNA library using 10x genomics protocols.
    • Use "Chromium Next GEM Singel Cell 3' Reagents Kits v3.1"
    • Access to the instrument or whole sample preparation.

       Details/docs/specs

    • Bio-fragment analyzer
    • For the quality check of mRNA samples  and DNA (10x libraries). Use as QC before sending for NGS.
    • 1 to 96 samples.
    • Sensitivity up to 5pg/ul.