Cytoviva hyperspectral microscope | acquired 2013
The nanocharacterization facility provides access to a diverse range of microscopes and scattering instruments essential for research in nanomaterials and related disciplines.
Light microscopes
-
Confocal Laser Scanning microscope
Acquired 2021
Leica Stellaris 5
Brand
Leica LSM Stellaris 5 | acquired 2021
Laserlines (all LEDs)
405 | 488 | 561 | 633
Objectives
- HC PL Fluotar 10x/0.32NA (Brightfield, DIC, Phase)
- HC PL Plan Apochromat 20x/0.75NA (Brightfield, DIC,)
- HC PL Plan Apochromat 63x/1.40NA oil (Brightfield, DIC,)
Scanheads
- Confocal point scanner with speed up to 2600 Hz: 10 frames / second @ 512 x 512
- Resonance scanner with speed up to 8000 Hz
Detectors
4 Hydrid detectors (HyD S)
Environmental
On stage Okolab | Heating unit | CO2 unit | Humidity control
-
Darkfield scattering microscope
Acquired 2014
Cytoviva
Brand
Light source
Dolan-jenner 420-1000nm | X-cite 120Q
Objectives
Plan-Achromat 10x/0.25 | UPL Fluorite 40x/0.75 | UPL Fluorite 100X/.6-1.30 Iris
Detection
SPECIM V10E Imaging spectrograph with a PCO pixelfly | QImaging Exi Blue
Fluoroscence
DAPI | FITC | Texas Red
-
Widefield microscope (brightfield and fluorescence)
Aquired 2011, refurbished 2024
Zeiss
Brand
Zeiss LSM 710 meta | acquired 2011 | refitted 2024
Laserlines (all LEDs)
405 | 488 | 561 | 630
Objectives
-
Plan-Apochromat 10x/0.3 M27
-
Plan-Apochromat 20x/0.8 M27
-
EC Plan-Neofluar 40x/1.30 Oil DIC M27
-
Plan-Apochromat 63x/1.4 Oil M27
Camera
Hamamatsu OrcaFlash4.0 V3
-
sCMOS technology
-
Cell size (“pixel”) 6.5 µm2
-
Pixel array: 2048 x 2048
-
Peak quantum efficiency: 82%@560nm
Number of pixels Frames per second 2048 100 1024 200 512 400 256 801 128 1603 64 3206 8 25 655 Environmental
Incubator XLmulti S1 | Heating unit XL S1 | CO2 unit S1
-
Electron microscopes
-
Scanning electron microscope
Acquired 2014
Tescan Mira 3
Brand
Tescan Mira3 LM FE | acquired 2014
Emittor
Field-emission Schottky emitter | High voltage: 0.5-30 kV
Detectors
- Everhart-Thornley type Secondary electron detector
- Retractable BSE detector with high sensitivity
- In-Beam SE Detector
- Energy-dispersive X-ray spectroscopy (EDX) detector, Peltier-cooled.
Technical
- Live stereoscopic imaging using 3D Beam Technology
- Full 5-axis motorized compucentric stage.Computized stage
-
Transmission electron microscope
Acquired 2014
Tecnai Spirit
Brand
Tecnai Spirit | acquired 2014
Features
BioTwin lens | 120 kV LaB6 emitter | 3Å resolution limit
Detectors
Veleta 2048×2048 wide angle | Eagle 4096×4096 bottom mount
Technical
Computized stage | Gatan cryoholder | Single tilt tomography
Condenser apertures 200 μm | 100 μm | 50 μm | 30 μm
Objective apertures 100 μm | 40 μm | 20 μm | 10 μm
-
Focused ion beam scanning electron microscope
Aqcuired 2018
ThermoFisher SCIOS2
Brand
ThermoFischer Scios 2 | acquired 2018
Electron optics
- Non-immersion column
- High-stability Schottky field emission gun at 20 eV-30 keV
- 0.9 nm resolution at 30 keV STEM, 1.2 nm at 1 keV with beam deceleration
Ion optics
- Sidewinder ion column with acceleration voltage: 500 V – 30 kV
- Beam current range: 1.5 pA – 65 nA
- Ion beam resolution: 3.0 nm at 30kV
Detectors
Up to four simultaneously detected signals
- Trinity Detection System (in-lens and in-column)
– T1 segmented lower in-lens detector
– T2 upper in-lens detector - Everhart-Thornley secondary electron detector
- Retractable low-voltage, high-contrast, segmented solid-state
backscatter electron detector - Retractable STEM 3+ detector with Brightfield/ Darkfield/ high angle annular darkfield
Stage
Flexible 5-axis motorized stage with compucentric rotation and tilt:
- XY range: 110 mm, Z range: 65 mm, endless rotation (360°)
- Tilt range: -15° to +60°
- Max sample height: Clearance 85 mm to eucentric point
- Max sample weight at 0° tilt: 2 kg (including sample holder)
- Max sample size: 110 mm with full rotation (larger samples
possible with limited rotation)
-
CryoTransmission electron microscopy
Acquired 2024
Leica GP2 cryoplunger
Prerequisite
proficiency in TEM, i.e. having passed the conventional TEM training and an additional 10-20 hours experience operating the TEM without help. Showcase your experience to the admin before enrolling in the cryoTEM training.
Atomic force microscopy
-
Park AFM
Acquired 2016
Park NX10Brand
Park systems NX10 AFM
Imaging modes
- Contact mode
- Tapping mode
- True non-contact mode (recommended for soft samples and minimal tip wear)
- Many other modes are available to measure electrical, mechanical, magnetic, thermal properties, and forces.
System features
- Enclosed in an acoustic hood and vibration isolation table.
- Independent XY scanner for sample positioning, decoupled from the Z scanner feedback loop, improving accuracy in nano resolution.
- XY scan range: 50 µm × 50 µm
- Z scan range: 15 µm
- Sample size: recommended ≤50 × 50 mm, max thickness 20 mm
- Motorized XY stage (20 mm × 20 mm, 0.6 µm steps) and motorized Z stage (25 mm travel, 0.08 µm steps)
- Magnetic sample holder
- Currently used only for imaging dry samples
-
Nanowizard AFM
Out of order
Dynamic light scattering
-
DLS instrument
Acquired 2012
LS Instruments
Brand
LS instruments
Modes
Static (SLS) | Dynamic light scattering (DLS) | depolarized DLS | 3D cross-correlation scattering (3D-DLS) for optically turbid samples.
Laser
Cobolt 05-01 diode pumped solid state laser
λ = 660 nm | P max. = 500 mW | linearly polarized and collimatedLight collection
- Single-mode optical fibres with integrated collimation optics and laser-line filters
- Scattering angles: 10° to 135°
- Q-range for aqueous suspensions: 2.2 106 to 2.3 106 m-1
- Two high-sensitivity APD detectors (Perkin Elmer, Single Photon Counting Module)
- Single processing via a two-channel multiple-tau correlator
Small Angle X-ray Scattering
-
Rigaku SAXS
Acquired 2009
Rigaku SAXS & WAXS
Source
Rigaku NanoMax-IQ
Source
Cu-α source (photon wavelength λ = 0.1524 nm)
Detection
SAXS/WAXS camera
Resolution
Accessible Q-range: 0.08 – 33.8 nm-1