Microscopes and data analysis workstations

The core facility offers access to a variety of different microscopes that usually are prevalent in a particular scientific domain. There are different setups that favour particular types of applications; for instance inverted microscope stands are generally used for live cell imaging.

Confocal systems can image an optical section through a translucent and fluorescently labeled sample. This can be used to get single high contrast images locations from relatively deep into the sample or for multiple consecutive sections for volume imaging. Confocal microscopes use either a point-wise scanner or a spinning disk, where the latter trades the capability to image deep into the sample for imaging speed.

Slide scanners focus on the throughput. Sample loading, scan area detection, determination of the focal plane and eventually the scanning can be fully automated, allowing to acquire images of whole slides with little operator supervision. The typical application cases are scanning of larger tissue sections, serial sections, and other types of sample collections.

There is a number of wide-field systems with special functionality, including Stereology - a robust way to quantify three dimensional structures by way of a set of sequential planar two dimensional sections - or total internal reflection fluorescense (TIRF ) microscopy to study small objects close to the cover glass at high lateral resolution.

Finally there we also provide a number of image processing workstations, specifically tailored to the microscopy data.

  • Laser scanning confocal
    • Inverted DMi8 stand, fully motorized
    • Diode laser: 405 nm
    • Pulsed white light laser 440-790 nm (1nm steps)
    • 4 HyD detectors (fluo)
    • 1 PMT transmitted light
    • Resonnant scanner 8kHz
    • Environment control (T, CO2, humidity)
    • Inverted DMI6000 stand, fully motorized
    • 3 PMT detectors (fluo)
    • 1 PMT transmitted light
    • AOBS
    • Resonnant scanner 4kHz
    • Lasers lines: 405, 458, 476, 488, 496, 514, 561, 633 nm
    • Upright DM6000CS stand, fully motorized
    • 3 PMT detectors (fluo)
    • 1 PMT transmitted light
    • Laser lines: 405, 458, 476, 488, 514, 543, 633 nm
    • Upright DM5500Q
    • Motorized stage
    • 1 PMT (fluo)
  • Spinning disk confocal
    • Nikon Ti-E inverted microscope
    • CSU-W1 Spinning Disk Head with 50um pinhole disk
    • Evolve 512 (Photometrics) EM-CDD camera (16um pixel size)
    • PCO.edge 4.2 sCMOS camera: (6.5 um)
    • Environmental sample chamber (temperature and CO2 control)
    • Photomanipulation module (FRAP, FRET, Photoactivation)
    • Laser ablation module
  • Slide scanner
    • Upright setup
    • Wide-field color imaging
    • Epi-fluorescence monochrome imaging
    • 60/30 capacity of standard/double glass slides
    • Upright setup
    • Wide-field color imaging
    • Epi-fluorescence monochrome imaging
    • Capacity to load 210 standard glass slides
    • Upright DM6B stand
    • Fully motorized
    • Large stage for multiple glass slides
    • High speed sCMOS
    • Color CMOS
    • LAS X Navigator

     

  • Wide-field microscope
    • Inverted DMI6000 stand with motorized stage (only z-direction)
    • DFC360FX CCD camera (1.4M pixels, 20 fps)
    • Fast filter wheels (for FRET)
    • FRET Wizard
    • Upright microscope
    • Halogen lamps for transmitted and reflected light
    • Fully motorized and computer controlled stage
    • Monochrome high resolution camera
    • StereoInvestigator software
  • Evanescent field microscope
    • Oympus IX71 inverted stand
    • CoolSnap HQ2 CCD camera
    • Fast excitation/emission filter wheels
    • Laser module for TIRF/FRAP
    • Hardware autofocus
    • Environment control (C02, humidity and temperature)
    • Integrated deconvolution
  • Data processing workstation
    • Large 32'' screen
    • High-end graphic card
    • Fast network: ~800 MB/sec
    • Large and Fast file storage (M.2 SSD)
    • As much RAM as the motherboard can hold (64-128 GB)