Microscopes and data analysis workstations
The core facility offers access to a variety of different microscopes that usually are prevalent in a particular scientific domain. There are different setups that favour particular types of applications; for instance inverted microscope stands are generally used for live cell imaging.
Confocal systems can image an optical section through a translucent and fluorescently labeled sample. This can be used to get single high contrast images locations from relatively deep into the sample or for multiple consecutive sections for volume imaging. Confocal microscopes use either a point-wise scanner or a spinning disk, where the latter trades the capability to image deep into the sample for imaging speed.
Slide scanners focus on the throughput. Sample loading, scan area detection, determination of the focal plane and eventually the scanning can be fully automated, allowing to acquire images of whole slides with little operator supervision. The typical application cases are scanning of larger tissue sections, serial sections, and other types of sample collections.
There is a number of wide-field systems with special functionality, including Stereology - a robust way to quantify three dimensional structures by way of a set of sequential planar two dimensional sections - or total internal reflection fluorescense (TIRF ) microscopy to study small objects close to the cover glass at high lateral resolution.
Finally there we also provide a number of image processing workstations, specifically tailored to the microscopy data.
Laser scanning confocal
Spinning disk confocal
- Nikon Ti-E inverted microscope
- CSU-W1 Spinning Disk Head with 50um pinhole disk
- Evolve 512 (Photometrics) EM-CDD camera (16um pixel size)
- PCO.edge 4.2 sCMOS camera: (6.5 um)
- Environmental sample chamber (temperature and CO2 control)
- Photomanipulation module (FRAP, FRET, Photoactivation)
- Laser ablation module
- Evanescent field microscope
- Data processing workstation