Microscopes and data analysis workstations
The core facility offers access to a variety of different microscopes that usually are prevalent in a particular scientific domain.
Laser scanning confocal
Spinning disk confocal
- Nikon Ti-E inverted microscope
- CSU-W1 Spinning Disk Head with 50um pinhole disk
- Evolve 512 (Photometrics) EM-CDD camera (16um pixel size)
- PCO.edge 4.2 sCMOS camera: (6.5 um)
- Environmental sample chamber (temperature and CO2 control)
- Photomanipulation module (FRAP, FRET, Photoactivation)
- Laser ablation module
- Live cell imager
- Evanescent field microscope
- Data processing workstation
Confocal systems can image an optical section through a translucent and fluorescently labeled sample. This can be used to get single high contrast images locations from relatively deep into the sample or for multiple consecutive sections for volume imaging. Confocal microscopes use either a point-wise scanner or a spinning disk, where the latter trades some of the capability to image deep into the sample for imaging speed.
Slide scanners focus on the throughput. Sample loading, scan area detection, determination of the focal plane and the scanning can be fully automated. The typical application cases are scanning of larger tissue sections, serial sections, and other types of sample collections.
Further (wide-field) applications include; a Stereology microscope allowing to quantify 1-3 dimensional structures by way of randomly sampling a set of sequential planar sections, a total internal reflection fluorescense (TIRF ) microscope to study small objects close to the cover glass at a high lateral resolution and a cell imager, to read out multiples measurements of single cells accros entire multiwell plates - also known as high content screening (HCS).
Most of the microscopes do not provide all software functionality to quantify the acquired data. For this reason, we also provide a number of image processing workstations, specifically tailored to the microscopy data.