Cryo-transmission electron microscopy
Leica GP2
Acquired 2023
Prerequisite
proficiency in TEM, i.e. having passed the conventional TEM training and an additional 10-20 hours experience operating the TEM without help. Showcase your experience to the admin before enrolling in the cryoTEM training.
Trainings
The training to perform cryofixation and cryo-TEM imaging is much more time-intensive than conventional TEM: a motivated participant may be able to learn cryo-TEM in about 25 hours over a period of 5-6 weeks, provided he/she brings proficiency in TEM. Training is conducted in small groups of no more than three participants. Note that grids are included in the training as cryo-TEM grids are different from conventional TEM grids. The correct type and supplier are discussed during the training.
Trainings are conducted twice per year – provided there is a need. Typically, the trainings take place in August – September and January – February. The following applies:
- TEM proficiency (TEM training and 10-20h in TEM use). See below
- Need for cryoTEM in a long-term project (> 1 year)
- The course comproses 7 to 8 sessions of 1/2 day, spread roughly 4 weeks.
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SNSF-eligible cost breakdown (training)
Hourly use Total Total costs Scientific support 83 CHF/h 25 h 2075 CHF Instrument costs (cryo) 33.70 CHF/h 25 h 842.50 CHF CryoTEM grids 5 CHF/piece 25 pieces 175 CHF Summary: Total costs for training: 3092.5 CHF. Total costs for a median group (2 people): 1546 CHF per person. Note: suitable samples, a contract longer than 1 year, and sufficient motivation are prerequisites for entering cryo-TEM training.
Output: the knowhow and handling of all the equipment to cryofix biological samples (or nanoparticle solutions in water) and the knowhow to image them.