Instruments

The Cell Analytics Facility provide full access to cutting-edge instruments of flow cytometry. It also contain some instrument to prepare library for NSG. 

Here is the list of the different instruments available in the Cell Analytics Facility at UNIFR. To access these instruments, please follow the process on the Cell Analytics Services page.

How to access instruments

  • Flow Cytometer Analysers

    UNIFR_CAF_Cytoflex_SOP

    Direct Booking (if access)

    • 4 lasers : 405nm (violet) / 488nm (blue) / 561nm (yellow-green) / 638nm (red)
    • Simultaneous detection of up to 13 fluorochromes + FSC/SSC
    • Single manual 5ml tube or 96-wells automatic plate loader options (U-V-deep well).
    • fluidics system using hydrodynamic focusing and sample injection via peristatic pump

    UNIFR_CAF_Fortessa_SOP

    Direct booking (if access)

    • 3 lasers : 405nm (violet) / 488nm (blue) / 640nm (red)
    • Simultaneous detection of up to 11 fluorochromes + FSC/SSC
    • Single manual 5ml tube loader
    • fluidics system using hydrodynamic focusing and sample injection via pressurisation
    • running under BD FACSDIVA 6.0 software

    UNIFR_CAF_Attune_SOP

    Direct booking (if access)

    • 4 lasers : 405nm (violet) / 488nm (blue) / 561 nm (yellow-green) / 640nm (red)
    • Simultaneous detection of up to 14 fluorochromes + FSC/SSC
    • Single manual 5ml tube or 96-wells automatic plate loader options (U-V-deep well).
    • Fluidics system using acoustic focusing and volumetrix counting of the sample injected via a pump
    • Running under Attune NxT software

    UNIFR_CAF_Aurora-SOP

    Direct booking (if access)

    • Full spectrum system 
    • 5 lasers : 355nm (UV) / 405nm (Violet) / 488nm (blue) / 561nm (Yellow-Green) / 640nm (red)
    • Simultaneous detection of many (theoretical maximum of 60) +FSC/2x SSC
    • Single manual 5ml tube loader
    • Loader for racks (40 tubes) or 96-wells plates (U, V, deep-well) automatic acquisition
    • Running under Spectroflo v.3.3.0
  • Flow Cytometer Cell Sorter
    • Our FACSAria Fusion is mainly dedicated to the isolation of eukaryotic nucleated cells. Yeast, bacteria, nanomaterial or any other special applications must be discussed with the facility staff first.
    • Any population of cells inside a more complex heterogeneous-population can be isolated, as long as the cells of interest can be identified in the parental population by light scattering and/or fluorescence (for example: GFP-infected cells or antigen-marker populations using fluoro-labeled antibodies).

       Details/docs/specs

    • 3 lasers : 405nm (violet 85mW) / 488nm (blue 50mW) / 640nm (red 100mW)
    • Simultaneous detection of up to 14 fluorochromes +FSC/SSC
    • Single tube loader
    • Sort of up to 4 independent cell populations in parallel.
    • Sort in 15ml or 5ml tubes, eppendorfs, slides or 384 to 6 wells plates. 
    • Operator used only!
    • Sterile (biosafety cabinet) and BSL-2 samples sort possible.
  • Sample preparation for NGS

       Details/docs/specs

    • For the preparation of single-cell mRNA library using 10x genomics protocols.
    • Use "Chromium Next GEM Singel Cell 3' Reagents Kits v3.1"
    • Access to the instrument or whole sample preparation.

    UNIFR_CAF_Qsep100_SOP

    Direct booking

    • Bio-fragment analyzer
    • For the quality check of mRNA samples  and DNA (10x libraries). Use as QC before sending for NGS.
    • 1 to 96 samples.
    • Sensitivity up to 5pg/ul.