Microscopes and data analysis workstations
The core facility offers access to a variety of different microscopes that usually are prevalent in a particular scientific domain.
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Multiphoton microscope
- Upright Bergamo II stand
- Epi-fluorescence: 5Mpix sCMOS
- Galvo scanner
- Resonant scanner
- Z-Piezzo (on objective)
- 2P Laser
- 3P Laser
-
Laser scanning confocal
- Inverted Axio Observer 7
- 8kHz tandem scanner
- Lasers (nm): 405, 488, 561, 639, 730
- Airyscan detector
- 4 GaAsP and 2 PMT spectral detectors
- Red-shifted GaAsP and GaAs far-red detectors
- T-PMT for transmitted light
- Lightfield 4D (instant volume imaging)
- Environment control (T, CO2, humidity)
- Inverted DMi8 stand, fully motorized
- Diode laser: 405 nm
- Pulsed white light laser 440-790 nm (1nm steps)
- 4 HyD detectors (fluo)
- T-PMT for transmitted light
- Galvo scanner 1-1400 Hz
- Resonnant scanner 8kHz
- Environment control (T, CO2, humidity)
- Inverted DMI6000 stand, fully motorized
- 4 HyD and1 PMT detectors (fluo)
- T-PMT for transmitted light
- AOBS
- Galvo scanner 1-1400 Hz
- Resonnant scanner 8kHz
- Lasers (nm): 405, 458, 476, 488, 496, 514, 561, 594, 633
- Upright DM6000CS stand, fully motorized
- 3 PMT detectors (fluo)
- T-PMT for transmitted light
- AOBS
- Galvo scanner 1-1400 Hz
- Resonnant scanner 8kHz
- Laser (nm): 405, 458, 476, 488, 496, 514, 543, 633
- Upright DM5500Q
- Motorized stage
- 1 PMT (fluo)
- Lasers (nm): 405, 488, 532, 635
-
Spinning disk confocal
- Nikon Ti-E inverted microscope
- CSU-W1 Spinning Disk Head with 50um pinhole disk
- Evolve 512 (Photometrics) EM-CDD camera (16um pixel size)
- PCO.edge 4.2 sCMOS camera: (6.5 um)
- Photomanipulation module (FRAP, FRET, Photoactivation)
- Laser ablation module
-
Whole slide scanner
- Upright setup
- Wide-field color imaging
- Epi-fluorescence monochrome imaging
- 60/30 capacity of standard/double glass slides
- Upright setup
- Wide-field color imaging
- Epi-fluorescence monochrome imaging
- Capacity to load 210 standard glass slides
- Upright DM6B stand
- Fully motorized
- Large stage for multiple glass slides
- High speed sCMOS
- Color CMOS
- LAS X Navigator
- StereoInvestigator software
-
Live cell imaging microplate reader
- Imaging modalities:
- Bright-field
- Epi-fluorescence
- Absorbance
- Laser auto-focus module
- Incubator (termperature, CO2, humidity) with 8 slots
- 6-, 12-, 24-, 48-, 96-, 384- and 1536-well microplate compatible
(petri dishes, slides and flasks with adapters)
- Imaging modalities:
-
Wide-field microscope
- Inverted DMi8 stand, fully motorized
- Hardware autofocus
- K8 Camera (95 frames/sec, read noise 1.1 e- (RMS), max. QE 95%)
- Fluorescence light source : LED 8 (wavelengths)
- Fully automated, large ITK quantum stage
(mult-slide multi-well plate inserts) - Integrated deconvolution and computational clearing
- Stage top chamber (T, CO2, humidity control)
-
Evanescent field microscope
- Oympus IX71 inverted stand
- CoolSnap HQ2 CCD camera
- Fast excitation/emission filter wheels
- Laser module for TIRF/FRAP
- Hardware autofocus
- Environment control (
C02, humidity and temperature) - Integrated deconvolution
-
Data processing workstation
- Large 32'' screen
- High-end graphic card
- Fast network: ~800 MB/sec
- Large and Fast file storage (M.2 SSD)
- As much RAM as the motherboard can hold (64-128 GB)